CultureBotAI · realmarcin · Sep 16, 2026
diff --git a/pages/structures/appendage/archaeal_cannula.html b/pages/structures/appendage/archaeal_cannula.html
@@ -40,7 +40,63 @@ <h2>Functions</h2>
 
 <h2>Mechanism graphs</h2>
 <h3 id="causal_graphs-calcium_dependent_cana_cannula_assembly">CanA coordinates calcium during cannula-fiber assembly <small>(ASSEMBLY)</small></h3>
-<p>Pyrodictium CanA binds calcium ions and undergoes donor-strand complementation to assemble hollow extracellular cannula fibers that extend from the cell envelope and participate in a cell-surface network.</p><table><thead><tr><th>Subject</th><th>Predicate</th><th>Object</th><th>Evidence</th></tr></thead><tbody>
+<p>Pyrodictium CanA binds calcium ions and undergoes donor-strand complementation to assemble hollow extracellular cannula fibers that extend from the cell envelope and participate in a cell-surface network.</p><figure class="mechanism-diagram" style="margin:1rem 0">
+<figcaption>Directed graph · arrows run from subject to object. Focus or hover over a node or arrow for details; the evidence table follows.</figcaption>
+<input type="checkbox" id="graph-7a62850e4df528b3-scale"/><label for="graph-7a62850e4df528b3-scale"> Show at full size (scroll to explore)</label>
+<style>#graph-7a62850e4df528b3-scale:checked ~ div svg {width:1644px;height:462px}</style>
+<div tabindex="0" role="region" aria-label="Scrollable mechanism graph" style="overflow:auto;max-height:75vh;border:1px solid #94a3b8;border-radius:8px;background:#f8fafc">
+<svg xmlns="http://www.w3.org/2000/svg" class="mechanism-svg" role="img" aria-labelledby="graph-7a62850e4df528b3-title graph-7a62850e4df528b3-desc" width="1644" height="462" viewBox="0 0 1644 462" style="display:block;max-width:none;font:13px system-ui,sans-serif;color:#172033">
+<title id="graph-7a62850e4df528b3-title">CanA coordinates calcium during cannula-fiber assembly</title>
+<desc id="graph-7a62850e4df528b3-desc">6 nodes and 5 directed relationships. Labels and evidence are also available in the adjacent table.</desc>
+<defs><marker id="graph-7a62850e4df528b3-arrow" markerWidth="10" markerHeight="8" refX="9" refY="4" orient="auto" markerUnits="userSpaceOnUse"><path d="M0,0 L10,4 L0,8 Z" fill="#475569"/></marker></defs>
+<style>.mechanism-svg{width:100%;height:auto}.mechanism-svg .graph-node:focus rect,.mechanism-svg .graph-node:hover rect{stroke:#0369a1;stroke-width:3}.mechanism-svg .graph-edge:focus path,.mechanism-svg .graph-edge:hover path{stroke:#0369a1;stroke-width:3}</style>
+<g class="graph-edge" tabindex="0" role="group" aria-label="CanA cannula glycoprotein — coordinates → calcium ion; Evidence: DOI:10.1038/s41467-025-64120-8" data-subject="cana_cannula_glycoprotein" data-object="calcium_ion">
+<title>CanA cannula glycoprotein — coordinates → calcium ion; Evidence: DOI:10.1038/s41467-025-64120-8</title><path d="M296,108.0 C386.0,108.0 386.0,108.0 476,108.0" fill="none" stroke="#475569" stroke-width="1.7" marker-end="url(#graph-7a62850e4df528b3-arrow)"/>
+<text x="386.0" y="98.0" text-anchor="middle" fill="#334155" style="paint-order:stroke;stroke:#f8fafc;stroke-width:4;stroke-linejoin:round">coordinates</text></g>
+<g class="graph-edge" tabindex="0" role="group" aria-label="CanA cannula glycoprotein — polymerizes into → hollow cannula fiber; Evidence: DOI:10.1038/s41467-025-64120-8; DOI:10.1038/s41598-025-13242-6" data-subject="cana_cannula_glycoprotein" data-object="hollow_cannula_fiber">
+<title>CanA cannula glycoprotein — polymerizes into → hollow cannula fiber; Evidence: DOI:10.1038/s41467-025-64120-8; DOI:10.1038/s41598-025-13242-6</title><path d="M296,108.0 C386.0,108.0 386.0,274.0 476,274.0" fill="none" stroke="#475569" stroke-width="1.7" marker-end="url(#graph-7a62850e4df528b3-arrow)"/>
+<text x="386.0" y="181.0" text-anchor="middle" fill="#334155" style="paint-order:stroke;stroke:#f8fafc;stroke-width:4;stroke-linejoin:round">polymerizes into</text></g>
+<g class="graph-edge" tabindex="0" role="group" aria-label="hollow cannula fiber — forms → archaeal cannula; Evidence: DOI:10.1016/S1047-8477(02)00581-6" data-subject="hollow_cannula_fiber" data-object="archaeal_cannula">
+<title>hollow cannula fiber — forms → archaeal cannula; Evidence: DOI:10.1016/S1047-8477(02)00581-6</title><path d="M712,274.0 C802.0,274.0 802.0,108.0 892,108.0" fill="none" stroke="#475569" stroke-width="1.7" marker-end="url(#graph-7a62850e4df528b3-arrow)"/>
+<text x="802.0" y="181.0" text-anchor="middle" fill="#334155" style="paint-order:stroke;stroke:#f8fafc;stroke-width:4;stroke-linejoin:round">forms</text></g>
+<g class="graph-edge" tabindex="0" role="group" aria-label="archaeal cannula — enters → Pyrodictium cell envelope; Evidence: DOI:10.1016/S1047-8477(02)00581-6" data-subject="archaeal_cannula" data-object="cell_envelope">
+<title>archaeal cannula — enters → Pyrodictium cell envelope; Evidence: DOI:10.1016/S1047-8477(02)00581-6</title><path d="M1128,108.0 C1218.0,108.0 1218.0,108.0 1308,108.0" fill="none" stroke="#475569" stroke-width="1.7" marker-end="url(#graph-7a62850e4df528b3-arrow)"/>
+<text x="1218.0" y="98.0" text-anchor="middle" fill="#334155" style="paint-order:stroke;stroke:#f8fafc;stroke-width:4;stroke-linejoin:round">enters</text></g>
+<g class="graph-edge" tabindex="0" role="group" aria-label="archaeal cannula — interconnects cells within → Pyrodictium cell-cell network; Evidence: DOI:10.1006/jsbi.1995.1032" data-subject="archaeal_cannula" data-object="cell_cell_network">
+<title>archaeal cannula — interconnects cells within → Pyrodictium cell-cell network; Evidence: DOI:10.1006/jsbi.1995.1032</title><path d="M1128,108.0 C1218.0,108.0 1218.0,274.0 1308,274.0" fill="none" stroke="#475569" stroke-width="1.7" marker-end="url(#graph-7a62850e4df528b3-arrow)"/>
+<text x="1218.0" y="181.0" text-anchor="middle" fill="#334155" style="paint-order:stroke;stroke:#f8fafc;stroke-width:4;stroke-linejoin:round">interconnects cells within</text></g>
+<g class="graph-node" tabindex="0" role="group" aria-label="CanA cannula glycoprotein (GENE_OR_PROTEIN); cana_cannula_glycoprotein" data-node-id="cana_cannula_glycoprotein"><title>CanA cannula glycoprotein (GENE_OR_PROTEIN); cana_cannula_glycoprotein</title>
+<rect x="60" y="70" width="236" height="76" rx="8" fill="#e0f2fe" stroke="#0369a1"/>
+<text x="72" y="89" fill="#075985" font-size="10">GENE_OR_PROTEIN</text>
+<text x="72" y="109" fill="#172033">CanA cannula glycoprotein</text>
+</g>
+<g class="graph-node" tabindex="0" role="group" aria-label="calcium ion (CHEMICAL); calcium_ion" data-node-id="calcium_ion"><title>calcium ion (CHEMICAL); calcium_ion</title>
+<rect x="476" y="70" width="236" height="76" rx="8" fill="#e0f2fe" stroke="#0369a1"/>
+<text x="488" y="89" fill="#075985" font-size="10">CHEMICAL</text>
+<text x="488" y="109" fill="#172033">calcium ion</text>
+</g>
+<g class="graph-node" tabindex="0" role="group" aria-label="hollow cannula fiber (STRUCTURE); hollow_cannula_fiber" data-node-id="hollow_cannula_fiber"><title>hollow cannula fiber (STRUCTURE); hollow_cannula_fiber</title>
+<rect x="476" y="236" width="236" height="76" rx="8" fill="#e0f2fe" stroke="#0369a1"/>
+<text x="488" y="255" fill="#075985" font-size="10">STRUCTURE</text>
+<text x="488" y="275" fill="#172033">hollow cannula fiber</text>
+</g>
+<g class="graph-node" tabindex="0" role="group" aria-label="Pyrodictium cell envelope (STRUCTURE); cell_envelope" data-node-id="cell_envelope"><title>Pyrodictium cell envelope (STRUCTURE); cell_envelope</title>
+<rect x="1308" y="70" width="236" height="76" rx="8" fill="#e0f2fe" stroke="#0369a1"/>
+<text x="1320" y="89" fill="#075985" font-size="10">STRUCTURE</text>
+<text x="1320" y="109" fill="#172033">Pyrodictium cell envelope</text>
+</g>
+<g class="graph-node" tabindex="0" role="group" aria-label="archaeal cannula (STRUCTURE); archaeal_cannula; cellstructuremech:archaeal_cannula" data-node-id="archaeal_cannula"><title>archaeal cannula (STRUCTURE); archaeal_cannula; cellstructuremech:archaeal_cannula</title>
+<rect x="892" y="70" width="236" height="76" rx="8" fill="#e0f2fe" stroke="#0369a1"/>
+<text x="904" y="89" fill="#075985" font-size="10">STRUCTURE</text>
+<text x="904" y="109" fill="#172033">archaeal cannula</text>
+</g>
+<g class="graph-node" tabindex="0" role="group" aria-label="Pyrodictium cell-cell network (STRUCTURE); cell_cell_network" data-node-id="cell_cell_network"><title>Pyrodictium cell-cell network (STRUCTURE); cell_cell_network</title>
+<rect x="1308" y="236" width="236" height="76" rx="8" fill="#e0f2fe" stroke="#0369a1"/>
+<text x="1320" y="255" fill="#075985" font-size="10">STRUCTURE</text>
+<text x="1320" y="275" fill="#172033">Pyrodictium cell-cell network</text>
+</g>
+</svg></div></figure>
+<table><thead><tr><th>Subject</th><th>Predicate</th><th>Object</th><th>Evidence</th></tr></thead><tbody>
 <tr><td>CanA cannula glycoprotein</td><td>coordinates</td><td>calcium ion</td><td><ul class="evidence"><li><a href="https://doi.org/10.1038/s41467-025-64120-8"><code>DOI:10.1038/s41467-025-64120-8</code></a> <small>Sleutel et al. 2025 resolved calcium-bound CanA interfaces in self-assembled archaeal cannulae.</small></li></ul></td></tr>
 <tr><td>CanA cannula glycoprotein</td><td>polymerizes into</td><td>hollow cannula fiber</td><td><ul class="evidence"><li><a href="https://doi.org/10.1038/s41467-025-64120-8"><code>DOI:10.1038/s41467-025-64120-8</code></a> <small>Sleutel et al. 2025 compared native and reassembled CanA cannula fibers by cryo-EM.</small></li><li><a href="https://doi.org/10.1038/s41598-025-13242-6"><code>DOI:10.1038/s41598-025-13242-6</code></a> <small>Munte et al. 2025 analyzed divalent-ion-induced CanA polymerization into hollow-fiber networks.</small></li></ul></td></tr>
 <tr><td>hollow cannula fiber</td><td>forms</td><td>archaeal cannula</td><td><ul class="evidence"><li><a href="https://doi.org/10.1016/S1047-8477(02)00581-6"><code>DOI:10.1016/S1047-8477(02)00581-6</code></a> <small>Nickell et al. 2003 resolved hollow Pyrodictium cannulae by cryo-electron tomography.</small></li></ul></td></tr>